molecular typing and phylogenetic analysis of some species belonging to phlebotomus (larroussius) and phlebotomus (adlerius) subgenera (diptera: psychodidae) from two locations in iran
نویسندگان
چکیده
background: haematophagous females of some phlebotomine sandflies are the only natural vectors of leishmania species, the causative agents of leishmaniasis in many parts of the tropics and subtropics, including iran. we report the presence of phlebotomus (larroussius) major and phlebotomus (adlerius) halepensis in tonekabon (mazanderan province) and phlebotomus (larroussius) tobbi in pakdasht (tehran province). it is the first report of these species, known as potential vectors of zoonotic visceral leishmaniasis in iran, are identified in these areas. methods: in 2006-2007 individual wild-caught sandflies were characterized by both morphological features and sequence analysis of their mitochondrial genes (cytochrome b). the analyses were based on a fragment of 494 bp at the 3´ end of the cyt b gene ( cyt b 3´ fragment) and a fragment of 382 bp cb3 at the 5´ end of the cyt b gene (cyt b 5´ fragment). we also analysed the cyt b long fragment, which is located on the last 717 bp of the cyt b gene, followed by 20 bp of intergenic spacer and the transfer rna ser(tcn) gene. results: twenty-seven p. halepensis and four p. major from dohezar, tonekabon, mazanderan province and 8 p. tobbi from packdasht, tehran province were identified by morphological and molecular characters. cyt b 5´ and cyt b 3´ fragment sequences were obtained from 15 and 9 flies, respectively. cyt b long fragment sequences were obtained from 8 out of 27 p. halepensis . conclusion: parsimony analyses (using heuristic searches) of the dna sequences of cyt b always showed monophyletic clades of subgenera and each species did form a monophyletic group.
منابع مشابه
Molecular Typing and Phylogenetic Analysis of Some Species Belonging to Phlebotomus (Larroussius) and Phlebotomus (Adlerius) Subgenera (Diptera: Psychodidae) from Two Locations in Iran
BACKGROUND Haematophagous females of some phlebotomine sandflies are the only natural vectors of Leishmania species, the causative agents of leishmaniasis in many parts of the tropics and subtropics, including Iran. We report the presence of Phlebotomus (Larroussius) major and Phlebotomus (Adlerius) halepensis in Tonekabon (Mazanderan Province) and Phlebotomus (Larroussius) tobbi in Pakdasht (T...
متن کاملPhylogenetic analysis of Phlebotomus species belonging to the subgenus Larroussius (Diptera, psychodidae) by ITS2 rDNA sequences.
In the genealogy of Phlebotomus (Diptera: Psychodidae), morphological analyses have indicated that the subgenus Larroussius is a monophyletic group which is most closely related to the subgenera Transphlebotomus and Adlerius. We conducted a phylogenetic analysis of the relationships among six representative species of the subgenus Larroussius and one species representatitive of the Phlebotomus ...
متن کاملMorphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran
BACKGROUND Female sand flies of subgenus Adlerius are considered as probable vectors of visceral leishmaniasis in Iran. The objective of this study was to determine the morphological and genotypic variations in the populations of this subgenus in the country. METHODS Sand flies collected using sticky traps from 17 provinces during 2008-2010. The morphometric measurements were conducted with a...
متن کاملmorphological and genotypic variations among the species of the subgenus adlerius (diptera: psychodidae, phlebotomus) in iran
background : female sand flies of subgenus adlerius are considered as probable vectors of visceral leishmaniasis in iran. the objective of this study was to determine the morphological and genotypic variations in the populations of this subgenus in the country. methods : sand flies collected using sticky traps from 17 provinces during 2008–2010. the morphometric measurements were conducted with...
متن کاملPhlebotomus (Larroussius) orientalis (Diptera: Psychodidae) as a probable secondary vector of visceral leishmaniasis in Kenya.
According to the World Health Organization’s reports, leishmaniases in humans are caused by 20 species of protozoa, belonging to the genus Leishmania1. The parasites are transmitted by the bite of a female tiny, 2–3 mm long insect vector, the phlebotomine sandfly1. Leishmaniasis endemic areas in Kenya are—West Pokot, Kitui, Machakos, Meru, Koibatek and Kajiado districts2–6. Kala-azar (Visceral ...
متن کاملSand Flies of the Subgenus Adlerius (Diptera: Psychodidae) in an Endemic Focus of Visceral Leishmaniasis and Introduction of Phlebotomus (Adlerius) comatus as a New Record for Iran
BACKGROUND Sand flies of subgenus Adlerius has a wide geographical distribution in Iran and are mostly found in wild form in mountainous areas. They are always considered as probable vectors of visceral leishmaniasis. The objective of this study was to determine the Adlerius species and its composition in an endemic focus of zoonotic visceral leishmaniasis in northwest of the country. METHODS...
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عنوان ژورنال:
journal of arthropod-borne diseasesجلد ۴، شماره ۲، صفحات ۱-۱۰
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